Cell Patterning for Liver Tissue Engineering via Dielectrophoretic Mechanisms

Sensors

Wan Nurlina Wan Yahya¹  Nahrizul Adib Kadri¹ 

[1] Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mails:

关键词: cell patterning;    dielectrophoresis;    tissue engineering;    hepatocytes;    liver;    artificial organ;    artificial liver;    engineered liver;    lab-on-a-chip;    BioMEMS liver;   

DOI  :  10.3390/s140711714

来源: mdpi

【 摘 要 】

Liver transplantation is the most common treatment for patients with end-stage liver failure. However, liver transplantation is greatly limited by a shortage of donors. Liver tissue engineering may offer an alternative by providing an implantable engineered liver. Currently, diverse types of engineering approaches for in vitro liver cell culture are available, including scaffold-based methods, microfluidic platforms, and micropatterning techniques. Active cell patterning via dielectrophoretic (DEP) force showed some advantages over other methods, including high speed, ease of handling, high precision and being label-free. This article summarizes liver function and regenerative mechanisms for better understanding in developing engineered liver. We then review recent advances in liver tissue engineering techniques and focus on DEP-based cell patterning, including microelectrode design and patterning configuration.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.