Generation of Hepatobiliary Cell Lineages from Human Induced Pluripotent Stem Cells: Applications in Disease Modeling and Drug Screening

International Journal of Molecular Sciences

Pier Giulio Conaldi¹  Roberto Di Gesù²  Mattia Pasqua²  Cinzia Maria Chinnici²  Maria Giovanna Francipane² 

[1] Dipartimento della Ricerca, IRCCS ISMETT, 90127 Palermo, Italy;Fondazione Ri.MED, 90133 Palermo, Italy;

关键词: iPSCs;    hepatocytes;    cholangiocytes;    disease modeling;    drug testing;    tissue engineering;   

DOI  :  10.3390/ijms22158227

来源: DOAJ

【 摘 要 】

The possibility to reproduce key tissue functions in vitro from induced pluripotent stem cells (iPSCs) is offering an incredible opportunity to gain better insight into biological mechanisms underlying development and disease, and a tool for the rapid screening of drug candidates. This review attempts to summarize recent strategies for specification of iPSCs towards hepatobiliary lineages —hepatocytes and cholangiocytes—and their use as platforms for disease modeling and drug testing. The application of different tissue-engineering methods to promote accurate and reliable readouts is discussed. Space is given to open questions, including to what extent these novel systems can be informative. Potential pathways for improvement are finally suggested.

【 授权许可】

Unknown