New Potentially Active Pyrazinamide Derivatives Synthesized Under Microwave Conditions

Molecules

Ondrej Jandourek²  Martin Dolezal²  Jiri Kunes⁵  Vladimir Kubicek¹  Pavla Paterova³  Matus Pesko⁶  Vladimir Buchta³  Katarina Kralova⁴ 

[1] Department of Biophysics and Physical Chemistry, Faculty of Pharmacy, Charles University in Prague, Heyrovskeho 1203, Hradec Kralove 50005, Czech Republic; E-Mail:;Department of Medicinal Chemistry and Drug Analysis, Faculty of Pharmacy, Charles University in Prague, Heyrovskeho 1203, Hradec Kralove 50005, Czech Republic; E-Mail:;Department of Biological and Medical Sciences, Faculty of Pharmacy, Charles University in Prague, Heyrovskeho 1203, Hradec Kralove 50005, Czech Republic; E-Mails:;Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynska Dolina CH-2, Bratislava 842 15, Slovakia; E-Mail:;Department of Inorganic and Organic Chemistry, Faculty of Pharmacy, Charles University in Prague, Heyrovskeho 1203, Hradec Kralove 50005, Czech Republic; E-Mail:;Department of Environmental Ecology, Faculty of Natural Sciences, Comenius University, Mlynska Dolina CH-2, Bratislava 842 15, Slovakia; E-Mail:

关键词: aminodehalogenation reaction;    antifungal activity;    inhibition of photosynthetic electron transport;    pyrazinamide;    microwave-assisted synthesis;    structure-activity relationships;   

DOI  :  10.3390/molecules19079318

来源: mdpi

【 摘 要 】

A series of 18 N-alkyl substituted 3-aminopyrazine-2-carboxamides was prepared in this work according to previously experimentally set and proven conditions using microwave assisted synthesis methodology. This approach for the aminodehalogenation reaction was chosen due to higher yields and shorter reaction times compared to organic reactions with conventional heating. Antimycobacterial, antibacterial, antifungal and photosynthetic electron transport (PET) inhibiting in vitro activities of these compounds were investigated. Experiments for the determination of lipophilicity were also performed. Only a small number of substances with alicyclic side chain showed activity against fungi which was the same or higher than standards and the biological efficacy of the compounds increased with rising lipophilicity. Nine pyrazinamide derivatives also inhibited PET in spinach chloroplasts and the IC₅₀ values of these compounds varied in the range from 14.3 to 1590.0 μmol/L. The inhibitory activity was connected not only with the lipophilicity, but also with the presence of secondary amine fragment bounded to the pyrazine ring. Structure-activity relationships are discussed as well.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.