DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide

International Journal of Molecular Sciences

Laura Baranello²  Fedor Kouzine²  Damian Wojtowicz³  Kairong Cui¹  Teresa M. Przytycka³  Keji Zhao¹ 

[1] Systems Biology Center, NHLBI/NIH, Bldg 10 Rm 7B06A, 10 Center Drive, Bethesda, MD 20892, USA; E-Mails:;Laboratory of Pathology, NCI/NIH, Bldg 10 Rm 2N106, 10 Center Drive, Bethesda, MD 20892, USA; E-Mails:;Computational Biology Branch, NCBI/NLM/NIH, Bldg 38a, 8600 Rockville Pike, Bethesda, MD 20894, USA; E-Mails:

关键词: topoisomerases;    DNA damage;    transcription;   

DOI  :  10.3390/ijms150713111

来源: mdpi

【 摘 要 】

Genomic DNA is under constant assault by endogenous and exogenous DNA damaging agents. DNA breakage can represent a major threat to genome integrity but can also be necessary for genome function. Here we present approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) at the genome-wide scale by two methods called DSB- and SSB-Seq, respectively. We tested these methods in human colon cancer cells and validated the results using the Topoisomerase II (Top2)-poisoning agent etoposide (ETO). Our results show that the combination of ETO treatment with break-mapping techniques is a powerful method to elaborate the pattern of Top2 enzymatic activity across the genome.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.