Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer

Nutrients

Zhiyu Zhang³  Chunhao Yu³  Chun-Feng Zhang³  Xiao-Hui Wu³  Xiao-Dong Wen³  Samantha Anderson³  Wei Du⁴  Wei-Hua Huang²  Shao-Ping Li¹  Chong-Zhi Wang³ 

[1] State Key Laboratory of Quality Research in Chinese Medicine, and Institute of Chinese Medical Sciences, University of Macau, Macao, China; E-Mail:;Institute of Clinical Pharmacology, Central South University, Changsha 410000, China; E-Mail:;Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA; E-Mails:;Ben May Department for Cancer Research, Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA; E-Mail:

关键词: Oplopanax horridus;    oplopantriol A;    OPT A;    chemoprevention;    colorectal cancer;    HCT-116;    SW-480;    apoptosis;    cell cycle;    tumor necrosis factors;    death receptor signaling pathway;   

DOI  :  10.3390/nu6072668

来源: mdpi

【 摘 要 】

Oplopanax horridus is a North American botanical that has received limited investigations. We previously isolated over a dozen of the constituents from O. horridus, and among them oplopantriol A (OPT A) is a novel compound. In this study, we firstly evaluated the in vivo chemoprevention activities of OPT A using the xenograft colon cancer mouse model. Our data showed that this compound significantly suppressed tumor growth with dose-related effects (p < 0.01). Next, we characterized the compound’s growth inhibitory effects in human colorectal cancer cell lines HCT-116 and SW-480. With OPT A treatment, these malignant cells were significantly inhibited in both a concentration- and time-dependent manner (both p < 0.01). The IC50 was approximately 5 µM for HCT-116 and 7 µM for SW-480 cells. OPT A significantly induced apoptosis and arrested the cell cycle at the G2/M phase. From further mechanism explorations, our data showed that OPT A significantly upregulated the expression of a cluster of genes, especially the tumor necrosis factor receptor family and caspase family, suggesting that the tumor necrosis factor-related apoptotic pathway plays a key role in OPT A induced apoptosis.

【 授权许可】

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.