Isolation and Characterization of an α-Glucosidase Inhibitor from Musa spp. (Baxijiao) Flowers

Molecules

Zhanwu Sheng¹  Haofu Dai³  Siyi Pan¹  Hui Wang²  Yingying Hu² 

[1] College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; E-Mails:;College of Food Science and Technology, Hainan University, Haikou 570228, China; E-Mails:;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, China

关键词: α-glucosidase;    inhibitor;    hyperglycemia;    flowers of Musa spp. (Baxijiao);   

DOI  :  10.3390/molecules190710563

来源: mdpi

【 摘 要 】

The use of α-glucosidase inhibitors is considered to be an effective strategy in the treatment of diabetes. Using a bioassay-guided fractionation technique, five Bacillus stearothermophilus α-glucosidase inhibitors were isolated from the flowers of Musa spp. (Baxijiao). Using NMR spectroscopy analysis they were identified as vanillic acid (1), ferulic acid (2), β-sitosterol (3), daucosterol (4) and 9-(4′-hydroxyphenyl)-2-methoxyphenalen-1-one (5). The half maximal inhibitory concentration (IC₅₀) values of compounds 1–5 were 2004.58, 1258.35, 283.67, 247.35 and 3.86 mg/L, respectively. Compared to a known α-glucosidase inhibitor (acarbose, IC₅₀ = 999.31 mg/L), compounds 3, 4 and 5 showed a strong α-glucosidase inhibitory effect. A Lineweaver-Burk plot indicated that compound 5 is a mixed-competitive inhibitor, while compounds 3 and 4 are competitive inhibitors. The inhibition constants (K_i) of compounds 3, 4 and 5 were 20.09, 2.34 and 4.40 mg/L, respectively. Taken together, these data show that the compounds 3, 4 and 5 are potent α-glucosidase inhibitors.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.