IN VITRO EFFECT OF TERPENOIDS - RICH EXTRACT OF Momordica charantia ON ALPHA GLUCOSIDASE ACTIVITY

Vitae

关键词: α-glucosidase;    terpenoids;    inhibitor;    inhibition;    hyperglycemia;    extract;   

DOI  :  10.17533/udea.vitae.v25n3a05

来源: DOAJ

【 摘 要 】

ABSTRACTBackground: Momordica charantia is a vine, native to tropics and subtropics that has found use in herbal medicine. In Nigeria, the plant is locally used in the treatment of diabetes.Objective:To investigate the inhibitory effect of terpenoid-rich extract from Momordica charantia on α-glucosidase activity.Method:The inhibitory effect of the terpenoid-rich extract from Momordica charantia on the activity of alpha-glucosidase from Saccharomyces cerevisiae as well as the determination of the mode of inhibition were investigated using in vitro assay methods.Results:The results revealed that the terpenoid-rich extract had a high IC50 of 1.60 mg/mL. The Lineweaver-Burk plot revealed that the extract exhibited partially mixed-type inhibition of α-glucosidase.Conclusions:It can be inferred from this study that although terpenoid-rich extract inhibited α-glucosidase, further purification will be necessary on the extract so as to increase its potency as an inhibitor of α-glucosidase. The action of the extract on α-glucosidase provided an insight into the probable mechanism of action of Momordica charantia in reducing hyperglycemia.

【 授权许可】

Unknown