Enhanced Response of a Proteinase K-Based Conductometric Biosensor Using Nanoparticles

Sensors

Wided Nouira⁴  Abderrazak Maaref²  Abdelhamid Elaissari³  Francis Vocanson⁵  Maryam Siadat¹ 

[1] LASC, ISEA, University of Metz, 7 Marconi Street, 57070 Metz, France; E-Mail:;Laboratory of Interfaces and Advanced Materials, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia; E-Mail:;University of Lyon, CNRS, UMR 5007, LAGEP-CPE, 43 Bd. 11 Novembre 1918, Villeurbanne, France; E-Mail:;Institute of Analytical Sciences, University of Lyon, La Doua Street, 5, 69622 Villeurbanne, France; E-Mail:;Hubert Curien Laboratory, University of Lyon, F-42023 Saint-Etienne, France; E-Mail:

关键词: gold nanoparticles;    magnetic nanoparticles;    proteinase K;    hydrolase;    conductometric transducers;    response enhancement;   

DOI  :  10.3390/s140713298

来源: mdpi

【 摘 要 】

Proteinases are involved in a multitude of important physiological processes, such as protein metabolism. For this reason, a conductometric enzyme biosensor based on proteinase K was developed using two types of nanoparticles (gold and magnetic). The enzyme was directly adsorbed on negatively charged nanoparticles and then deposited and cross-linked on a planar interdigitated electrode (IDE). The biosensor was characterized with bovine serum albumin (BSA) as a standard protein. Higher sensitivity was obtained using gold nanoparticles. The linear range for BSA determination was then from 0.5 to 10 mg/L with a maximum response of 154 μs. These results are greater than that found without any nanoparticles (maximum response of 10 μs). The limit of detection (LOD) was 0.3 mg/L. An inter-sensor reproducibility of 3.5% was obtained.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.