Transcriptional Responses of the Bdtf1-Deletion Mutant to the Phytoalexin Brassinin in the Necrotrophic Fungus Alternaria brassicicola

Molecules

Yangrae Cho²  Robin A. Ohm¹  Rakshit Devappa²  Hyang Burm Lee³  Igor V. Grigoriev¹  Bo Yeon Kim² 

[1] Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek 94598, CA, USA; E-Mails:;Korea Research Institute of Bioscience and Biotechnology, Ochang, Chungbuk 363-883, Korea; E-Mail:;Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Buk-Gu, Gwangju 500-757, Korea; E-Mail:

关键词: phytoalexin;    brassinin;    gene expression profiles;    RNA-seq;    necrotrophic fungus;    phytoalexin detoxification;    GEO Series Accession No. GSE59195;   

DOI  :  10.3390/molecules190810717

来源: mdpi

【 摘 要 】

Brassica species produce the antifungal indolyl compounds brassinin and its derivatives, during microbial infection. The fungal pathogen Alternaria brassicicola detoxifies brassinin and possibly its derivatives. This ability is an important property for the successful infection of brassicaceous plants. Previously, we identified a transcription factor, Bdtf1, essential for the detoxification of brassinin and full virulence. To discover genes that encode putative brassinin-digesting enzymes, we compared gene expression profiles between a mutant strain of the transcription factor and wild-type A. brassicicola under two different experimental conditions. A total of 170 and 388 genes were expressed at higher levels in the mutants than the wild type during the infection of host plants and saprophytic growth in the presence of brassinin, respectively. In contrast, 93 and 560 genes were expressed, respectively, at lower levels in the mutant than the wild type under the two conditions. Fifteen of these genes were expressed at lower levels in the mutant than in the wild type under both conditions. These genes were assumed to be important for the detoxification of brassinin and included Bdtf1 and 10 putative enzymes. This list of genes provides a resource for the discovery of enzyme-coding genes important in the chemical modification of brassinin.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.