Characterization and Antitumor Activity of a Polysaccharide from Sarcodia ceylonensis

Molecules

Yijun Fan²  Mengchuan Lin²  Aoshuang Luo¹  Ze Chun¹ 

[1] Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China

关键词: Sarcodia ceylonensis;    polysaccharide;    antitumor;    purification;   

DOI  :  10.3390/molecules190810863

来源: mdpi

【 摘 要 】

A water-soluble polysaccharide from Sarcodia ceylonensis was obtained by using the method of water-extraction and ethanol-precipitation. The polysaccharide was further purified by chromatography on AB-8 and ADS-7 columns, yielding a pure polysaccharide termed SCP-60. The molecular weight (Mw) of SCP-60 was calculated to be 50.0 kDa, based on the calibration curve obtained with a series of Dextran T standards. The results of FT-IR indicated that the polysaccharide contains the α-configuration of sugar units. GC-MS analysis revealed that SCP-60 was mainly composed of galactose and glucose. NMR spectroscopy revealed SCP-60 had the backbone consisting of →6)-α-Manp-(1→, α-d-Glcp-(1→, →6)-α-d-Glcp-(1→ and →6)-α-Galp-(1→. In order to evaluate the antitumor activity in vivo of the polysaccharide, a sarcoma 180 model was used. The results showed SCP-60 had strong antitumor ability, meanwhile, SCP-60 at a high dose (100 mg/kg) could significantly increase the thymic and splenic indices of S180 mice, and strongly promote the secretion of IL-2, TNF-α and IFN-γ, increase the SOD activities and reduce the concentrations of MDA in blood. Therefore the polysaccharide SCP-60 should be explored as a novel potential antitumor drug.

【 授权许可】

CC BY   
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