Synthesis of Water-Dispersed Ferrecene/Phenylboronic Acid-Modified Bifunctional Gold Nanoparticles and the Application in Biosensing

Materials

Yun Xing²  Lin Liu¹  Danqing Zhao²  Yixin Yang² 

[1] College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, Henan, China;

关键词: gold nanoparticles;    ferrecene;    boronic acid;    biosensors;    diols;   

DOI  :  10.3390/ma7085554

来源: mdpi

【 摘 要 】

Phenylboronic acids can form tight covalent bonds with diol-containing biomolecules. In this work, water-dispersed bifunctional gold nanoparticles (AuNPs) modified with ferrecene (Fc)-derivatized peptides and 4-mercaptophenylboronic acids (MBA) (denoted as Fc–MBA–AuNPs) were synthesized and characterized by UV/vis spectrometry and transmission electron microscopy. To demonstrate the application and the analytical merits of the nanoparticles in biosensing, glycoprotein avidin was tested as a model analyte. Specifically, avidin was captured by the biotin-covered gold electrode via the strong biotin-avidin interaction. Then, Fc–MBA–AuNPs were attached by the captured avidin through the formation of tight covalent bonds between the boronic acid moieties of Fc–MBA–AuNPs and the oligosaccharides of avidin. As a result, a detection limit of 0.2 pM was achieved. We believe that the bifunctional nanoparticles would found many applications in amplified detection of diol-containing species by rational design of the surface chemistry of electrode.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.