Endophytic Fungi from Lycium chinense Mill and Characterization of Two New Korean Records of Colletotrichum

International Journal of Molecular Sciences

Narayan Chandra Paul²  Hyang Burm Lee²  Ji Hye Lee³  Kyu Seop Shin³  Tae Hee Ryu³  Hye Ri Kwon³  Yeong Kuk Kim¹  Young Nam Youn³ 

[1]Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration (RDA), Eumseung 369-873, Korea

[2] E-Mail:

[3]Division of Applied Bioscience & Biotechnology, College of Agriculture & Life Sciences, Chonnam National University, Gwangju 500-757, Korea

[4] E-Mails:

[5]Department of Agricultural Biology, College of Agriculture & Life Sciences, Chungnam National University, Daejeon 305-764, Korea

[6] E-Mails:

关键词: colletotrichum brevisporum;    colletotrichum fructicola;    endophytic fungi;    lycium chinense mill;    new records;   

DOI  :  10.3390/ijms150915272

来源: mdpi

【 摘 要 】

Chinese boxthorn or matrimony vine (Lycium chinense Mill) is found primarily in southeastern Europe and Asia, including Korea. The dried ripe fruits are commonly used as oriental medicinal purposes. Endophytic fungi were isolated from surface sterilized tissues and fruits of the medicinal plant in 2013 to identify the new or unreported species in Korea. Among 14 isolates, 10 morphospecies were selected for molecular identification with the internal transcribed spacer (ITS) gene. Phylogenetic analysis revealed that all isolates belonged to Ascomycota including the genera Acremonium, Colletotrichum, Cochliobolus, Fusarium, Hypocrea and Nemania. Two Colletotrichum species were identified at the species level, using three genes including internal transcribed spacer (ITS), glycerol-3-phosphate dehydrogenase (GAPDH) and Actin (ACT) for PCR and molecular data analysis along with morphological observations. The fungal isolates, CNU122031 and CNU122032 were identified as Colletotrichum fructicola and C. brevisporum, respectively. Morphological observations also well supported the molecular identification. C. brevisporum is represented unrecorded species in Korea and C. fructicola is the first record from the host plant.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.