Enzyme Molecules in Solitary Confinement

Molecules

Raphaela B. Liebherr¹ 

[1] id="af1-molecules-19-14417">Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg 93040, Germa

关键词: enzyme kinetics;    single molecule enzymology;    fluorescence microscopy;    femtoliter array;   

DOI  :  10.3390/molecules190914417

来源: mdpi

【 摘 要 】

Large arrays of homogeneous microwells each defining a femtoliter volume are a versatile platform for monitoring the substrate turnover of many individual enzyme molecules in parallel. The high degree of parallelization enables the analysis of a statistically representative enzyme population. Enclosing individual enzyme molecules in microwells does not require any surface immobilization step and enables the kinetic investigation of enzymes free in solution. This review describes various microwell array formats and explores their applications for the detection and investigation of single enzyme molecules. The development of new fabrication techniques and sensitive detection methods drives the field of single molecule enzymology. Here, we introduce recent progress in single enzyme molecule analysis in microwell arrays and discuss the challenges and opportunities.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.