Molecules | |
Glucuronoyl Esterase Screening and Characterization Assays Utilizing Commercially Available Benzyl Glucuronic Acid Ester | |
Hampus Sunner1  Maria-Despoina Charavgi2  Lisbeth Olsson1  Evangelos Topakas2  Paul Christakopoulos3  | |
[1] Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg SE-412 96, Sweden; E-Mails:;Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str., Zografou Campus, Athens 15780, Greece; E-Mails:;Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, Luleå SE-971 87, Sweden | |
关键词: glucuronic acid; glucuronoyl esterase; enzymatic assay; benzyl glucuronic acid ester; enzyme kinetics; Michaelis-Menten parameter estimation; | |
DOI : 10.3390/molecules201017807 | |
来源: mdpi | |
【 摘 要 】
Research on glucuronoyl esterases (GEs) has been hampered by the lack of enzyme assays based on easily obtainable substrates. While benzyl
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202003190005685ZK.pdf | 1832KB | download |