Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract

Molecules

Neelesh Sharma⁵  Kalpa W. Samarakoon¹  Rajendra Gyawali⁶  Yang-Ho Park²  Sung-Jin Lee³  Sung Jong Oh⁵  Tae-Hoon Lee⁴ 

[1] Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea; E-Mail:;BRM Institute, Seoul 135-010, Korea; E-Mail:;Department of Animal Biotechnology, School of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, Korea; E-Mail:;Department of Oral Biochemistry, Dental Science Research Institute, Chonnam National University, Gwangju 500-757, Korea; E-Mail:;Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju 690-756, Korea; E-Mails:;Faculty of Biotechnology, School of Applied Life Science, Jeju National University, Jeju 690-756, Korea; E-Mail:

关键词: Euphorbia hirta;    chemical composition;    online HPLC-ABTS^+• bioassay;    antioxidant;    anti-inflammatory;    anticancer;   

DOI  :  10.3390/molecules190914567

来源: mdpi

【 摘 要 】

This study evaluated the chemical composition, antioxidant, anti-inflammatory and anticancer activities of a Euphorbia hirta L. extract. The antioxidant activities of whole E. hirta ethanol extract were determined by electron spin resonance spectrophotometric analysis of 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl, and alkyl radical levels and by using an online high-performance liquid chromatography (HPLC)-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay. The E. hirta ethanol extract (0.5 mg/mL) exhibited DPPH-scavenging activity of 61.19% ± 0.22%, while the positive control (0.5 mg/mL ascorbic acid) had 100% ± 0.22% activity. The concentration of the extract required to trap 50% of DPPH (IC₅₀) was 0.205 mg/mL. Online HPLC analysis of the extract also showed strong antioxidant activity. The anti-inflammatory activity of the E. hirta extract was assessed in lipopolysaccharide-induced RAW 264.7 macrophages. The anti-inflammatory activity was highest in the presence of 200 µg/mL E. hirta extract, and nitric oxide production was decreased significantly (p < 0.05). The extract also showed selective anticancer activity at a concentration of 100 µg/mL (p < 0.05). These results indicated that E. hirta may warrant further investigation for the development of antioxidant, anti-inflammatory, and anticancer herbal medications.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.