Application of an Online-Biomass Sensor in an Optical Multisensory Platform Prototype for Growth Monitoring of Biotechnical Relevant Microorganism and Cell Lines in Single-Use Shake Flasks

Sensors

Christian Ude¹  Jörg Schmidt-Hager¹  Michael Findeis²  Gernot Thomas John²  Thomas Scheper¹ 

[1] Leibniz University of Hanover, Institute of Technical Chemistry, Callinstr. 5, 30167 Hannover, Germany; E-Mails:;PreSens Precision Sensing GmbH, Josef-Engert-Str. 11, 93053 Regensburg, Germany; E-Mails:

关键词: biomass sensor;    light scattering;    optical density;    process monitoring;    shake flask;   

DOI  :  10.3390/s140917390

来源: mdpi

【 摘 要 】

In the context of this work we evaluated a multisensory, noninvasive prototype platform for shake flask cultivations by monitoring three basic parameters (pH, pO₂ and biomass). The focus lies on the evaluation of the biomass sensor based on backward light scattering. The application spectrum was expanded to four new organisms in addition to E. coli K12 and S. cerevisiae [1]. It could be shown that the sensor is appropriate for a wide range of standard microorganisms, e.g., L. zeae, K. pastoris, A. niger and CHO-K1. The biomass sensor signal could successfully be correlated and calibrated with well-known measurement methods like OD_600, cell dry weight (CDW) and cell concentration. Logarithmic and Bleasdale-Nelder derived functions were adequate for data fitting. Measurements at low cell concentrations proved to be critical in terms of a high signal to noise ratio, but the integration of a custom made light shade in the shake flask improved these measurements significantly. This sensor based measurement method has a high potential to initiate a new generation of online bioprocess monitoring. Metabolic studies will particularly benefit from the multisensory data acquisition. The sensor is already used in labscale experiments for shake flask cultivations.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.