Optical Ammonia Sensor for Continuous Bioprocess Monitoring

Proceedings

Borisov, Sergey M.¹  Maierhofer, Maximilian²  Mayr, Torsten³ 

[1] Author to whom correspondence should be addressed.;Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, 8010 Graz, Austria;Presented at the Eurosensors 2018 Conference, Graz, Austria, 9–12 September 2018.

关键词: ammonia;    optical sensor;    process monitoring;    fluorescent dye;   

DOI  :  10.3390/proceedings2131041

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

We present an optical ammonia sensor suitable for bioprocess monitoring. A fluorescent dye is physically entrapped in a polyurethane hydrogel forming an emulsion system with vinylterminated polydimethylsiloxane (PDMS). The sensing layer is covered by a hydrophobic porous membrane which excludes hydrophilic substances. Ammonia, diffuses through this barrier and PDMS to the protonated dye, whereby it deprotonates the dye and switches off its emission. Readout is performed with a miniaturized phase fluorimeter combined with optical fibers. Dual-lifetime referencing (DLR) acts as detection method and Egyptian blue as reference material.

【 授权许可】

CC BY