Fluorescence-Based Bioassays for the Detection and Evaluation of Food Materials

Sensors

Kentaro Nishi²  Shin-Ichiro Isobe²  Yun Zhu¹  Ryoiti Kiyama¹ 

[1] Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan; E-Mail:;Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kyushu Sangyo University, 2-3-1 Matsukadai, Kasi-i, Higashi-ku, Fukuoka 813-8503, Japan; E-Mails:

关键词: fluorescent dye;    bioassay;    food study;    microarray;    signaling pathway;   

DOI  :  10.3390/s151025831

来源: mdpi

【 摘 要 】

We summarize here the recent progress in fluorescence-based bioassays for the detection and evaluation of food materials by focusing on fluorescent dyes used in bioassays and applications of these assays for food safety, quality and efficacy. Fluorescent dyes have been used in various bioassays, such as biosensing, cell assay, energy transfer-based assay, probing, protein/immunological assay and microarray/biochip assay. Among the arrays used in microarray/biochip assay, fluorescence-based microarrays/biochips, such as antibody/protein microarrays, bead/suspension arrays, capillary/sensor arrays, DNA microarrays/polymerase chain reaction (PCR)-based arrays, glycan/lectin arrays, immunoassay/enzyme-linked immunosorbent assay (ELISA)-based arrays, microfluidic chips and tissue arrays, have been developed and used for the assessment of allergy/poisoning/toxicity, contamination and efficacy/mechanism, and quality control/safety. DNA microarray assays have been used widely for food safety and quality as well as searches for active components. DNA microarray-based gene expression profiling may be useful for such purposes due to its advantages in the evaluation of pathway-based intracellular signaling in response to food materials.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.