Effect of Bacteriophage Infection in Combination with Tobramycin on the Emergence of Resistance in Escherichia coli and Pseudomonas aeruginosa Biofilms

Viruses

Lindsey B. Coulter¹  Robert J. C. McLean²  Rodney E. Rohde¹  Gary M. Aron² 

[1] Clinical Laboratory Science Program, Texas State University, 601 University Drive, San Marcos, TX 78666, USA; E-Mails:;Department of Biology, Texas State University, San Marcos, 601 University Drive, TX 78666, USA; E-Mail:

关键词: biofilms;    bacteriophage;    antibiotic;    resistance;    mixed therapy;   

DOI  :  10.3390/v6103778

来源: mdpi

【 摘 要 】

Bacteriophage infection and antibiotics used individually to reduce biofilm mass often result in the emergence of significant levels of phage and antibiotic resistant cells. In contrast, combination therapy in Escherichia coli biofilms employing T4 phage and tobramycin resulted in greater than 99% and 39% reduction in antibiotic and phage resistant cells, respectively. In P. aeruginosa biofilms, combination therapy resulted in a 60% and 99% reduction in antibiotic and PB-1 phage resistant cells, respectively. Although the combined treatment resulted in greater reduction of E. coli CFUs compared to the use of antibiotic alone, infection of P. aeruginosa biofilms with PB-1 in the presence of tobramycin was only as effective in the reduction of CFUs as the use of antibiotic alone. The study demonstrated phage infection in combination with tobramycin can significantly reduce the emergence of antibiotic and phage resistant cells in both E. coli and P. aeruginosa biofilms, however, a reduction in biomass was dependent on the phage-host system.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.