Anti-Inflammatory Effect of 3-O-[(6'-O-Palmitoyl)-β-d-glucopyranosyl Sitosterol] from Agave angustifolia on Ear Edema in Mice

Molecules

Elizabeth Hernández-Valle¹  Maribel Herrera-Ruiz¹  Gabriela Rosas Salgado³  Alejandro Zamilpa¹  Martha Lucia Arenas Ocampo²  Antonio Jiménez Aparicio²  Jaime Tortoriello¹  Enrique Jiménez-Ferrer¹ 

[1] Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social (IMSS), Argentina No. 1, Xochitepec, Morelos CP 62790, Mexico; E-Mails:;Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional (IPN), Km 6 carr. Yautepec-Jojutla, calle Ceprobi No. 6 col. San Isidro, Yautepec, Morelos CP 62731, Mexico; E-Mails:;Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Leñeros s/n. Col. Volcanes, Cuernavaca, Morelos CP 62350, Mexico; E-Mail:

关键词: Agave angustifolia;    3-O-[(6'-O-palmitoyl)-β-d-glucopyranosyl] sitosterol;    anti-inflammatory;   

DOI  :  10.3390/molecules191015624

来源: mdpi

【 摘 要 】

In Mexico Agave angustifolia has traditionally been used to treat inflammation. The aim of this study was to measure the anti-inflammatory effect of the extract of A. angustifolia, the isolation and identification of active compounds. From the acetone extract two active fractions were obtained, (AsF13 and AaF16). For the characterization of pharmacological activity, the acute inflammatory model of mouse ear edema induced with TPA was used. The tissue exposed to TPA and treatments were subjected to two analysis, cytokine quantification (IL-1β, IL-6, IL-10 and TNF-α) and histopathological evaluation. The active fraction (AaF16) consisted principally of 3-O-[(6'-O-palmitoyl)-β-d-glucopyranpsyl] sitosterol. In AaF13 fraction was identified β-sitosteryl glucoside (2) and stigmasterol (3). The three treatments tested showed a concentration-dependent anti-inflammatory effect (AaAc E_max = 33.10%, EC₅₀ = 0.126 mg/ear; AaF13 E_max = 54.22%, EC₅₀ = 0.0524 mg/ear; AaF16 E_max = 61.01%, EC₅₀ = 0.050 mg/ear). The application of TPA caused a significant increase on level of IL-1β, IL-6 and TNFα compared with basal condition, which was countered by any of the experimental treatments. Moreover, the experimental treatments induced a significant increase in the levels of IL-4 and IL-10, compared to the level observed when stimulated with TPA. Therefore, the anti-inflammatory effect of Agave angustifolia, is associated with the presence of 3-O-[(6'-O-palmitoyl)-β-d-glucopyranosyl] sitosterol.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.