The Effect of Freeze/Thaw Cycles on Reproducibility of Metabolic Profiling of Marine Microalgal Extracts Using Direct Infusion High-Resolution Mass Spectrometry (HR-MS)

Molecules

Hans Chr. Eilertsen³  Siv Huseby¹  Maria Degerlund³  Gunilla K. Eriksen³  Richard A. Ingebrigtsen³  Espen Hansen² 

[1]MabCent-SFI, UiT The Arctic University of Norway, Tromsø N-9037, Norway

[2] E-Mail:

[3]Marbio, UiT The Arctic University of Norway, Tromsø N-9037, Norway

[4]Department of Arctic and Marine Biology, Faculty of Biosciences, Fisheries and Economics, UiT The Arctic University of Norway, Tromsø N-9037, Norway

[5] E-Mails:

关键词: extracts;    metabolic profiling;    mass spectrometry;    freeze/thaw cycles;    stability;   

DOI  :  10.3390/molecules191016373

来源: mdpi

【 摘 要 】

During normal sample preparation, storage in freezers and subsequent freeze/thaw cycles are commonly introduced. The effect of freeze/thaw cycles on the metabolic profiling of microalgal extracts using HR-MS was investigated. Methanolic extracts of monocultures of Arctic marine diatoms were analyzed immediately after extraction, after seven days of storage at −78 °C (one freeze/thaw cycle), and after additional seven days at −20 °C (two freeze/thaw cycles). Repeated direct infusion high-resolution mass spectrometry analysis of microalgae extracts of the same sample showed that reproducibility was ca. 90% when a fresh (unfrozen) sample was analyzed. The overall reproducibility decreased further by ca. 10% after the first freeze/thaw-cycle, and after one more freeze/thaw cycle the reproducibility decreased further by ca. 7%. The decrease in reproducibility after freeze-thaw cycles could be attributed to sample degradation and not to instrument variability.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.