Preparation and Evaluation of Human-Murine Chimeric Antibody against Protective Antigen of Bacillus anthracis

International Journal of Molecular Sciences

Lina Hao²  Feng Zheng²  Siping Xiong¹  Dan Hu²  Heng Lv²  Qi Tang¹  Jin Yang¹  Zhenqing Feng¹  Changjun Wang²  Jin Zhu² 

[1] Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing 210029, China; E-Mails:;Huadong Medical Institute of Biotechniques, Nanjing 210002, China; E-Mails:

关键词: chimeric Fab antibody;    anthrax;    PA;   

DOI  :  10.3390/ijms151018496

来源: mdpi

【 摘 要 】

The aim of this research is to develop a human/murine chimeric Fab antibody which neutralizes the anthrax toxin, protective antigen (PA). The chimeric Fab was constructed using variable regions of murine anti-PA monoclonal antibody in combination with constant regions of human IgG. The chimeric PA6-Fab was expressed in E. coli. BL21 and evaluated by ELISA and co-immunoprecipitation- mass spectra. The potency of PA6-Fab to neutralize LeTx was examined in J774A.1 cell viability in vitro and in Fisher 344 rats in vivo. The PA6-Fab did not have domain similarity corresponding to the current anti PA mAbs, but specifically bound to anthrax PA at an affinity of 1.76 nM, and was able to neutralize LeTx in vitro and protected 56.9% cells at 20 μg/mL against anthrax LeTx. One hundred μg PA6-Fab could neutralize 300 μg LeTx in vivo. The PA6-Fab has potential as a therapeutic mAb for treatment of anthrax.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.