Laboratory Studies on Surface Sampling of Bacillus anthracis Contamination: Summary, Gaps, and Recommendations

Piepel, Gregory F. ; Amidan, Brett G. ; Hu, Rebecca

关键词: BACILLUS;    CLEARANCE;    CONTAMINATION;    MANAGEMENT;    PERFORMANCE;    PLATES;    PROCESSING;    RECOMMENDATIONS;    SAMPLING;    SURFACE CONTAMINATION Bacillus anthracis;    anthrax;    surface sampling;    recovery efficiency;    false negative rate;    limit of detection;   

DOI  :  10.2172/1031439
RP-ID  :  PNNL-20910
PID  :  OSTI ID: 1031439
Others  :  Other: 400904120
Others  :  TRN: US201201%%707

学科分类：生物科学（综合）

美国|英语

来源: SciTech Connect

【 摘 要 】

This report summarizes previous laboratory studies to characterize the performance of methods for collecting, storing/transporting, processing, and analyzing samples from surfaces contaminated by Bacillus anthracis or related surrogates. The focus is on plate culture and count estimates of surface contamination for swab, wipe, and vacuum samples of porous and nonporous surfaces. Summaries of the previous studies and their results were assessed to identify gaps in information needed as inputs to calculate key parameters critical to risk management in biothreat incidents. One key parameter is the number of samples needed to make characterization or clearance decisions with specified statistical confidence. Other key parameters include the ability to calculate, following contamination incidents, the (1) estimates of Bacillus anthracis contamination, as well as the bias and uncertainties in the estimates, and (2) confidence in characterization and clearance decisions for contaminated or decontaminated buildings. Gaps in knowledge and understanding identified during the summary of the studies are discussed and recommendations are given for future studies.