Bioconversion of Rebaudioside I from Rebaudioside A

Molecules

Indra Prakash¹  Cynthia Bunders¹  Krishna P. Devkota⁴  Romila D. Charan⁴  Catherine Ramirez⁴  Tara M. Snyder⁴  Christopher Priedemann⁴  Avetik Markosyan²  Cyrille Jarrin³  Robert Ter Halle³ 

[1] The Coca-Cola Company, Atlanta, GA 30313, USA; E-Mail:;PureCircle Limited, Lengkuk Teknologi, Bandar Enstek 71760 Malaysia; E-Mail:;Libragen SA, 3 Rue des Satellites, Toulouse 31400, France; E-Mails:;AMRI-Albany, Analytical Development, Albany, NY 12212, USA; E-Mails:

关键词: Stevia rebaudiana;    structure characterization;    bioconversion;    rebaudioside I;    rebaudioside A;   

DOI  :  10.3390/molecules191117345

来源: mdpi

【 摘 要 】

To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy (¹H, ¹³C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data to fully characterize rebaudioside I.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.