A Coumarin-Based Fluorescent Probe as a Central Nervous System Disease Biomarker

Sensors

Ann-Chee Yap²  Ummi Affah Mahamad¹  Shen-Yang Lim¹  Hae-Jo Kim³ 

[1] Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mails:;Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mail:;Department of Chemistry, Hankuk University of Foreign Studies, Yongin 449-791, Korea; E-Mail:

关键词: fluorescent probe;    fluorescence spectroscopy;    mass spectrometry;    homocysteine;    methylmalonic acid;    neurochemistry;    chemoassay;   

DOI  :  10.3390/s141121140

来源: mdpi

【 摘 要 】

Homocysteine and methylmalonic acid are important biomarkers for diseases associated with an impaired central nervous system (CNS). A new chemoassay utilizing coumarin-based fluorescent probe 1 to detect the levels of homocysteine is successfully implemented using Parkinson's disease (PD) patients' blood serum. In addition, a rapid identification of homocysteine and methylmalonic acid levels in blood serum of PD patients was also performed using the liquid chromatography-mass spectrometry (LC-MS). The results obtained from both analyses were in agreement. The new chemoassay utilizing coumarin-based fluorescent probe 1 offers a cost- and time-effective method to identify the biomarkers in CNS patients.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.