International Journal of Molecular Sciences | |
Growth Suppression of Colorectal Cancer by Plant-Derived Multiple mAb CO17-1A × BR55 via Inhibition of ERK1/2 Phosphorylation | |
Dong Hoon Kwak1  Ghislain Moussavou4  Ju Hyoung Lee4  Sung Youn Heo4  Kisung Ko2  Kyung-A Hwang5  Seung-Joo Jekal3  Young-Kug Choo1  | |
[1] Institute of Glycoscience, Wonkwang University, Iksan, Jeonbuk 570-749, Korea; E-Mail:;Department of Medicine, Medical Research Institute, College of Medicine Chung-Ang University, Heukseok-ro 84, Seoul 156-756, Korea; E-Mail:;Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan 570-750, Korea; E-Mail:;Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk 570-749, Korea; E-Mails:;Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon 441-853, Korea; E-Mail: | |
关键词: anti-EpCAM; colon cancer; mAbP CO17-1A × BR5; apoptosis; mAbP CO17-1A; monoclonal antibody; | |
DOI : 10.3390/ijms151121105 | |
来源: mdpi | |
【 摘 要 】
We have generated the transgenic Tabaco plants expressing multiple monoclonal antibody (mAb) CO7-1A × BR55 by cross-pollinating with mAb CO17-1A and mAb BR55. We have demonstrated the anti-cancer effect of plant-derived multiple mAb CO17-1A × BR55. We find that co-treatment of colorectal mAbs (anti-epithelial cellular adhesion molecule (EpCAM), plant-derived monoclonal antibody (mAbP) CO17-1A and mAbP CO17-1A × BR55) with RAW264.7 cells significantly inhibited the cell growth in SW620 cancer cells. In particular, multi mAbP CO17-1A × BR55 significantly and efficiently suppressed the growth of SW620 cancer cells compared to another mAbs. Apoptotic death-positive cells were significantly increased in the mAbP CO17-1A × BR55-treated. The mAbP CO17-1A × BR55 treatment significantly decreased the expression of B-Cell lymphoma-2 (BCl-2), but the expression of Bcl-2-associated X protein (Bax), and cleaved caspase-3 were markedly increased.
【 授权许可】
CC BY
© 2014 by the authors; licensee MDPI, Basel, Switzerland.
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