Growth Suppression of Colorectal Cancer by Plant-Derived Multiple mAb CO17-1A × BR55 via Inhibition of ERK1/2 Phosphorylation

International Journal of Molecular Sciences

Dong Hoon Kwak¹  Ghislain Moussavou⁴  Ju Hyoung Lee⁴  Sung Youn Heo⁴  Kisung Ko²  Kyung-A Hwang⁵  Seung-Joo Jekal³  Young-Kug Choo¹ 

[1] Institute of Glycoscience, Wonkwang University, Iksan, Jeonbuk 570-749, Korea; E-Mail:;Department of Medicine, Medical Research Institute, College of Medicine Chung-Ang University, Heukseok-ro 84, Seoul 156-756, Korea; E-Mail:;Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan 570-750, Korea; E-Mail:;Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk 570-749, Korea; E-Mails:;Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon 441-853, Korea; E-Mail:

关键词: anti-EpCAM;    colon cancer;    mAb^P CO17-1A × BR5;    apoptosis;    mAb^P CO17-1A;    monoclonal antibody;   

DOI  :  10.3390/ijms151121105

来源: mdpi

【 摘 要 】

We have generated the transgenic Tabaco plants expressing multiple monoclonal antibody (mAb) CO7-1A × BR55 by cross-pollinating with mAb CO17-1A and mAb BR55. We have demonstrated the anti-cancer effect of plant-derived multiple mAb CO17-1A × BR55. We find that co-treatment of colorectal mAbs (anti-epithelial cellular adhesion molecule (EpCAM), plant-derived monoclonal antibody (mAb^P) CO17-1A and mAb^P CO17-1A × BR55) with RAW264.7 cells significantly inhibited the cell growth in SW620 cancer cells. In particular, multi mAb^P CO17-1A × BR55 significantly and efficiently suppressed the growth of SW620 cancer cells compared to another mAbs. Apoptotic death-positive cells were significantly increased in the mAb^P CO17-1A × BR55-treated. The mAb^P CO17-1A × BR55 treatment significantly decreased the expression of B-Cell lymphoma-2 (BCl-2), but the expression of Bcl-2-associated X protein (Bax), and cleaved caspase-3 were markedly increased. In vivo, the mAb^P CO17-1A × BR55 significantly and efficiently inhibited the growth of colon tumors compared to another mAbs. The apoptotic cell death and inhibition of pro-apoptotic proteins expression were highest by treatment with mAb^P CO17-1A × BR55. In addition, the mAb^P CO17-1A × BR55 significantly inhibited the extracellular signal-regulated kinase 1 and 2 (ERK1/2) phosphorylation in cancer cells and tumors. Therefore, this study results suggest that multiple mAb^P CO17-1A × BR55 has a significant effect on apoptosis-mediated anticancer by suppression of ERK1/2 phosphorylation in colon cancer compared to another mAbs. In light of these results, further clinical investigation should be conducted on mAb^P CO17-1A × BR55 to determine its possible chemopreventive and/or therapeutic efficacy against human colon cancer.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.