Antifungal Compounds Produced by Colletotrichum gloeosporioides, an Endophytic Fungus from Michelia champaca

Molecules

Vanessa Mara Chapla³  Maria Luiza Zeraik³  Ioanis Hcristos Leptokarydis³  Geraldo Humberto Silva⁴  Vanderlan Silva Bolzani³  Maria Claudia M. Young²  Ludwig Heinrich Pfenning¹  Angela Regina Araújo³ 

[1] Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, MG, 37200-000, Brazil; E-Mail:;Instituto de Botânica, Núcleo de Pesquisa em Fisiologia e Bioquímica, São Paulo-SP, 04301-902, Brazil; E-Mail:;NuBBE - Núcleo de Bioensaios, Biossíntese e Ecofisiologia de Produtos Naturais, Departamento de Química Orgânica, Instituto de Química, UNESP, Universidade Estadual Paulista, Araraquara-SP 14800-900, Brazil; E-Mails:;Instituto de Ciências Exatas, Universidade Federal de Viçosa, Viçosa, MG, 38810-000, Brazil; E-Mail:

关键词: Colletotrichum gloeosporioides;    endophytic fungus;    antifungal activity;   

DOI  :  10.3390/molecules191119243

来源: mdpi

【 摘 要 】

In this study, eight endophytic fungi were isolated from the leaves, stems and roots of Michelia champaca. The isolates were screened and evaluated for their antifungal, anticancer and acetylcholinesterase (AChE) inhibitory activities. All of the extracts exhibited potent activity against two evaluated phytopathogenic fungi. Chemical investigation of EtOAc extracts of the endophytic fungus Colletotrichum gloeosporioides resulted in the isolation of one new compound, 2-phenylethyl 1H-indol-3-yl-acetate (1), and seven known compounds: uracil (2), cyclo-(S*-Pro-S*-Tyr) (3), cyclo-(S*-Pro-S*-Val) (4), 2(2-aminophenyl)acetic acid (5), 2(4-hydroxyphenyl)acetic acid (6), 4-hydroxy- benzamide (7) and 2(2-hydroxyphenyl)acetic acid (8). All of the compound structures were elucidated using 1D and 2D NMR and MS analyses. The antifungal and AChE inhibitory activities of compounds 1–8 were evaluated in vitro. Compound 1 exhibited promising activity against Cladosporium cladosporioides and C. sphaerospermum that was comparable to that of the positive control nystatin.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.