Antifungal activity of metabolites of the endophytic fungus Trichoderma brevicompactum from garlic

Brazilian Journal of Microbiology

Xuping Shentu²  Xiaohuan Zhan¹  Zheng Ma¹  Xiaoping Yu¹  Chuanxi Zhang² 

[1] ,Zhejiang University Institute of Insect Science Hangzhou,China

关键词: Trichoderma brevicompactum;    garlic;    endophytic fungus;    Trichodermin;    antifungal activity;   

DOI  :  10.1590/S1517-83822014005000036

来源: SciELO

【 摘 要 】

The endophytic fungus strain 0248, isolated from garlic, was identified as Trichoderma brevicompactum based on morphological characteristics and the nucleotide sequences of ITS1-5.8SITS2 and tef1. The bioactive compound T2 was isolated from the culture extracts of this fungus by bioactivity-guided fractionation and identified as 4β-acetoxy-12,13-epoxy-Δ9-trichothecene (trichodermin) by spectral analysis and mass spectrometry. Trichodermin has a marked inhibitory activity on Rhizoctonia solani, with an EC50 of 0.25 µgmL-1. Strong inhibition by trichodermin was also found for Botrytis cinerea, with an EC50 of 2.02 µgmL-1. However, a relatively poor inhibitory effect was observed for trichodermin against Colletotrichum lindemuthianum (EC50 = 25.60 µgmL-1). Compared with the positive control Carbendazim, trichodermin showed a strong antifungal activity on the above phytopathogens. There is little known about endophytes from garlic. This paper studied in detail the identification of endophytic T. brevicompactum from garlic and the characterization of its active metabolite trichodermin.

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