Polyphenolic Profile, Antioxidant and Anti-Inflammatory Activity of Eastern Teaberry (Gaultheria procumbens L.) Leaf Extracts

Molecules

Piotr Michel²  Anna Dobrowolska²  Agnieszka Kicel²  Aleksandra Owczarek²  Agnieszka Bazylko¹  Sebastian Granica¹  Jakub P. Piwowarski¹  Monika A. Olszewska² 

[1] Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Warsaw Medical University, 1 Banacha St., Warsaw 02-097, Poland; E-Mails:;Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, 1 Muszyńskiego St., Lodz 90-151, Poland; E-Mails:

关键词: Gaultheria procumbens L.;    antioxidant activity;    anti-inflammatory activity;    UHPLC-MS;    HPLC-PDA;    phenolic content;    phenolic profile;    proanthocyanidins;    chlorogenic acids;    flavonoids;   

DOI  :  10.3390/molecules191220498

来源: mdpi

【 摘 要 】

Dry leaf extracts of eastern teaberry (Gaultheria procumbens L.) were evaluated as a source of bioactive phytocompounds through systematic activity testing and phytochemical profiling. The antioxidant efficiency was tested using five complementary in vitro models (DPPH; FRAP; linoleic acid (LA) peroxidation assay; O₂^•− and H₂O₂ scavenging tests) in parallel with standard antioxidants. The 75% methanol extract and its diethyl ether, ethyl acetate (EAF), n-butanol and water fractions exhibited the dose-dependent responses in all assays, with the highest capacities found for EAF (DPPH EC₅₀ = 2.9 μg/mL; FRAP = 12.8 mmol Fe²⁺/g; IC₅₀ for LA-peroxidation = 123.9 μg/mL; O₂^•− SC₅₀ = 3.9 μg/mL; H₂O₂ SC₅₀ = 7.2 μg/mL). The EAF had also the highest anti-inflammatory activity in the inhibition tests of lipoxygenase and hyaluronidase (60.14% and 21.83% effects, respectively, at the concentration of 100 μg/mL). Activity parameters of the extracts correlated strongly with the levels of total phenolics (72.4–270.7 mg GAE/g), procyanidins, and phenolic acids, whereas for flavonoids only moderate effects were observed. Comprehensive UHPLC-PDA-ESI-MS³ and HPLC-PDA studies led to the identification of 35 polyphenols with a procyanidin A-type trimer, quercetin 3-O-glucuronide, isomers of caffeoylquinic acids, and (‒)-epicatechin being the dominant components. Significant activity levels, high phenolic contents and high extraction yields (39.4%–42.5% DW for defatted and crude methanol extracts, respectively) indicate the value of eastern teaberry leaves as bioactive products.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.