Nrf2-Mediated HO-1 Induction Contributes to Antioxidant Capacity of a Schisandrae Fructus Ethanol Extract in C2C12 Myoblasts

Nutrients

Ji Sook Kang³  Min Ho Han⁴  Gi-Young Kim²  Cheol Min Kim¹  Byung Woo Kim³  Hye Jin Hwang³ 

[1] Department of Biochemistry, College of Medicine, Busan National University, Yangsan 626-870, Korea; E-Mail:;Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Korea; E-Mail:;Blue-Bio Industry Regional Innovation Center andAnti-Aging Research Center, Dongeui University, Busan 614-714, Korea; E-Mails:;Department of Biochemistry, College of Korean Medicine, Dongeui University, Busan 614-052, Korea; E-Mail:

关键词: Schisandrae Fructus;    oxidative stress;    ROS;    Nrf2/HO-1;   

DOI  :  10.3390/nu6125667

来源: mdpi

【 摘 要 】

This study was designed to confirm the protective effect of Schisandrae Fructus, which are the dried fruits of Schisandra chinensis (Turcz.) Baill, against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms in C2C12 myoblasts. Preincubating C2C12 cells with a Schisandrae Fructus ethanol extract (SFEE) significantly attenuated hydrogen peroxide (H₂O₂)-induced inhibition of growth and induced scavenging activity against intracellular reactive oxygen species (ROS) induced by H₂O₂. SFEE also inhibited comet tail formation and phospho-histone γH2A.X expression, suggesting that it prevents H₂O₂-induced cellular DNA damage. Furthermore, treating C2C12 cells with SFEE significantly induced heme oxygenase-1 (HO-1) and phosphorylation of nuclear factor-erythroid 2 related factor 2 (Nrf2). However, zinc protoporphyrin IX, a potent inhibitor of HO-1 activity, significantly reversed the protective effects of SFEE against H₂O₂-induced growth inhibition and ROS generation in C2C12 cells. Additional experiments revealed that the potential of the SFEE to induce HO-1 expression and protect against H₂O₂-mediated cellular damage was abrogated by transient transfection with Nrf2-specific small interfering RNA, suggesting that the SFEE protected C2C12 cells against oxidative stress-induced injury through the Nrf2/HO-1 pathway.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.