Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries

International Journal of Molecular Sciences

Wenjin Cao²  Bisheng Fu²  Kun Wu²  Na Li²  Yan Zhou²  Zhongxia Gao²  Musen Lin²  Guoqiang Li²   Xinyi Wu²  Zhengqiang Ma²  Haiyan Jia¹ 

[1] College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China;

关键词: bacterial artificial chromosome;    wheat;    descending pool;   

DOI  :  10.3390/ijms151221896

来源: mdpi

【 摘 要 】

We have constructed three bacterial artificial chromosome (BAC) libraries of wheat cultivar Triticum aestivum Wangshuibai, germplasms T. monococcum TA2026 and TA2033. A total of 1,233,792,170,880 and 263,040 clones were picked and arrayed in 384-well plates. On the basis of genome sizes of 16.8 Gb for hexaploid wheat and 5.6 Gb for diploid wheat, the three libraries represented 9.05-, 2.60-, and 3.71-fold coverage of the haploid genomes, respectively. An improved descending pooling system for BAC libraries screening was established. This improved strategy can save 80% of the time and 68% of polymerase chain reaction (PCR) with the same successful rate as the universal 6D pooling strategy.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.