Towards the Fecal Metabolome Derived from Moderate Red Wine Intake

Metabolites

Ana Jiménez-Girón¹  Irene Muñoz-González²  Pedro J. Martín-Álvarez²  Mar໚ Victoria Moreno-Arribas²  Bego༚ Bartolomé² 

[1] Institute of Food Science Research (CIAL), CSIC-UAM, C/Nicolás Cabrera, 9, Campus de Cantoblanco, 28049 Madrid, Spain;

关键词: wine;    polyphenols;    fecal metabolome;    UPLC-ESI-MS/MS;    UHPLC-TOF MS;   

DOI  :  10.3390/metabo4041101

来源: mdpi

【 摘 要 】

Dietary polyphenols, including red wine phenolic compounds, are extensively metabolized during their passage through the gastrointestinal tract; and their biological effects at the gut level (i.e., anti-inflammatory activity, microbiota modulation, interaction with cells, among others) seem to be due more to their microbial-derived metabolites rather than to the original forms found in food. In an effort to improve our understanding of the biological effects that phenolic compounds exert at the gut level, this paper summarizes the changes observed in the human fecal metabolome after an intervention study consisting of a daily consumption of 250 mL of wine during four weeks by healthy volunteers (n = 33). It assembles data from two analytical approaches: (1) UPLC-ESI-MS/MS analysis of phenolic metabolites in fecal solutions (targeted analysis); and (2) UHPLC-TOF MS analysis of the fecal solutions (non-targeted analysis). Both approaches revealed statistically-significant changes in the concentration of several metabolites as a consequence of the wine intake. Similarity and complementarity between targeted and non-targeted approaches in the analysis of the fecal metabolome are discussed. Both strategies allowed the definition of a complex metabolic profile derived from wine intake. Likewise, the identification of endogenous markers could lead to new hypotheses to unravel the relationship between moderate wine consumption and the metabolic functionality of gut microbiota.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.