Progressive Adaptation of a CpGV Isolate to Codling Moth Populations Resistant to CpGV-M

Viruses

Benoît Graillot²  Marie Berling²  Christine Blachere-López¹  Myriam Siegwart⁴  Samantha Besse³  Miguel López-Ferber² 

[1] INRA, 6, Avenue de Clavières, 30319 Alès, France; E-Mail:;LGEI, Ecole des Mines d’Alès, Institut Mines-Telecom et Université de Montpellier Sud de France, 6, Avenue de Clavières, 30319 Alès, France; E-Mails:;Natural Plant Protection, Arysta LifeScience group, Avenue Léon Blum, 64000 Pau, France; E-Mail:;INRA, Unité PSH, Agroparc, 84914 Avignon Cedex 9, France; E-Mail:

关键词: Cydia pomonella granulovirus;    coddling moth;    resistance development;    biological control;   

DOI  :  10.3390/v6125135

来源: mdpi

【 摘 要 】

The NPP-R1 isolate of CpGV is able to replicate on CpGV-M-resistant codling moths. However, its efficacy is not sufficient to provide acceptable levels of control in natural (orchard) conditions. A laboratory colony derived from resistant codling moths was established, which exhibited a homogeneous genetic background and a resistance level more than 7000 fold. By successive cycles of replication of NPP-R1 in this colony, we observed a progressive increase in efficacy. After 16 cycles (isolate 2016-r16), the efficacy of the virus isolate was equivalent to that of CpGV-M on susceptible insects. This isolate was able to control both CpGV-M-susceptible and CpGV-M-resistant insects with similar efficacy. No reduction in the levels of occlusion body production in susceptible larvae was observed for 2016-r16 compared to CpGV-M.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.