Isolation and Characterization of Six AP2/ERF Transcription Factor Genes in Chrysanthemum nankingense

International Journal of Molecular Sciences

Chunyan Gao¹  Peiling Li¹  Aiping Song¹  Haibin Wang¹  Yinjie Wang¹  Liping Ren¹  Xiangyu Qi¹  Fadi Chen¹  Jiafu Jiang¹  Sumei Chen¹ 

[1] College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; E-Mails:

关键词: hormone;    PCR;    stress response;    transcription pattern;   

DOI  :  10.3390/ijms16012052

来源: mdpi

【 摘 要 】

The AP2/ERF family of plant transcription factors (TFs) regulate a variety of developmental and physiological processes. Here, we report the isolation of six AP2/ERF TF family genes from Chrysanthemum nankingense. On the basis of sequence similarity, one of these belonged to the Ethylene Responsive Factor (ERF) subfamily and the other five to the Dehydration Responsive Element Binding protein (DREB) subfamily. A transient expression experiment showed that all six AP2/ERF proteins localized to the nucleus. A yeast-one hybrid assay demonstrated that CnDREB1-1, 1-2 and 1-3 all function as transactivators, while CnERF1, CnDREB3-1 and 3-2 have no transcriptional activation ability. The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress. The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees. CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.

【 授权许可】

CC BY   
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