Artificial Affinity Proteins as Ligands of Immunoglobulins

Biomolecules

Barbara Mouratou¹  Ghislaine Béhar¹ 

[1] INSERM UMR 892 - CRCNA, 8 quai Moncousu, BP 70721, 44007 Nantes Cedex 1, France

关键词: immunoglobulin;    Fc;    alternative scaffold protein;    Affibody;    Affitin;    DARPin;    monobody;    knottin;    CBM;   

DOI  :  10.3390/biom5010060

来源: mdpi

【 摘 要 】

A number of natural proteins are known to have affinity and specificity for immunoglobulins. Some of them are widely used as reagents for detection or capture applications, such as Protein G and Protein A. However, these natural proteins have a defined spectrum of recognition that may not fit specific needs. With the development of combinatorial protein engineering and selection techniques, it has become possible to design artificial affinity proteins with the desired properties. These proteins, termed alternative scaffold proteins, are most often chosen for their stability, ease of engineering and cost-efficient recombinant production in bacteria. In this review, we focus on alternative scaffold proteins for which immunoglobulin binders have been identified and characterized.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.