Gold Nanoparticles Surface Plasmon Resonance Enhanced Signal for the Detection of Small Molecules on Split-Aptamer Microarrays (Small Molecules Detection from Split-Aptamers)

Microarrays

Feriel Melaine¹  Yoann Roupioz¹  Arnaud Buhot¹ 

[1] Univ. Grenoble Alpes, INAC-SPRAM, F-38000 Grenoble, France; E-Mails:

关键词: split-aptamers;    Surface Plasmon Resonance imaging;    gold nanoparticles;    small molecules detection;   

DOI  :  10.3390/microarrays4010041

来源: mdpi

【 摘 要 】

The detection of small molecules by biosensors remains a challenge for diagnostics in many areas like pharmacology, environment or homeland security. The main difficulty comes from both the low molecular weight and low concentrations of most targets, which generally requires an indirect detection with an amplification or a sandwich procedure. In this study, we combine both strategies as the amplification of Surface Plasmon Resonance imaging (SPRi) signal is obtained by the use of gold nanoparticles and the sequence engineering of split-aptamers, short oligonucleotides strands with strong affinity towards small targets, allows for a sandwich structure. Combining those two strategies, we obtained state-of-the-art results in the limit of detection (LOD = 50 nM) with the model target adenosine. Furthermore, the SPRi detection led on aptamer microarrays paves the way for potential multi-target detections thanks to the multi-probe imaging approach.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.