Applicability of Next Generation Sequencing Technology in Microsatellite Instability Testing

Genes

Chun Gan²  Clare Love²  Victoria Beshay³  Finlay Macrae¹  Stephen Fox³  Paul Waring²  Graham Taylor² 

[1] Department of Colorectal Medicine and Genetics, Familial Cancer Clinic, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia; E-Mail:;Department of Pathology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, Victoria 3052, Australia; E-Mails:;Department of Pathology and Sir Peter MacCallum Department of Oncology, University of Melbourne, East Melbourne, Victoria 3002, Australia; E-Mails:

关键词: microsatellite instability;    next generation sequencing;    colorectal cancer;    MiSeq;   

DOI  :  10.3390/genes6010046

来源: mdpi

【 摘 要 】

Microsatellite instability (MSI) is a useful marker for risk assessment, prediction of chemotherapy responsiveness and prognosis in patients with colorectal cancer. Here, we describe a next generation sequencing approach for MSI testing using the MiSeq platform. Different from other MSI capturing strategies that are based on targeted gene capture, we utilize “deep resequencing”, where we focus the sequencing on only the microsatellite regions of interest. We sequenced a series of 44 colorectal tumours with normal controls for five MSI loci (BAT25, BAT26, BAT34c4, D18S55, D5S346) and a second series of six colorectal tumours (no control) with two mononucleotide loci (BAT25, BAT26). In the first series, we were able to determine 17 MSI-High, 1 MSI-Low and 26 microsatellite stable (MSS) tumours. In the second series, there were three MSI-High and three MSS tumours. Although there was some variation within individual markers, this NGS method produced the same overall MSI status for each tumour, as obtained with the traditional multiplex PCR-based method.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.