Improved Osteoblast and Chondrocyte Adhesion and Viability by Surface-Modified Ti6Al4V Alloy with Anodized TiO2 Nanotubes Using a Super-Oxidative Solution

Materials

Ernesto Beltrán-Partida⁴  Aldo Moreno-Ulloa²  Benjamín Valdez-Salas³  Cristina Velasquillo¹  Monica Carrillo³  Alan Escamilla³  Ernesto Valdez⁵  Francisco Villarreal² 

[1] Instituto Nacional de Rehabilitación, Calz. México Xochimilco, No. 289, Arenal de Guadalupe, México C.P. 14389, D.F., Mexico; E-Mail:;School of Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA; E-Mails:;Instituto de Ingeniería, Universidad Autónoma de Baja California, Blvd. B. Juárez y Calle de la Normal s/n, Mexicali C.P. 21280, Baja California, Mexico; E-Mails:;Facultad de Odontología Mexicali, Universidad Autónoma de Baja California, Av. Zotoluca y Chinampas, s/n, Mexicali C.P. 21040, Baja California, Mexico; E-Mail:;Centro Medico Ixchel, Bravo y Obregón, Mexicali C.P. 21000, Baja California, Mexico; E-Mail:

关键词: titanium;    TiO₂ nanotubes;    chondrocyte;    osteoblasts;    adhesion;    tissue engineering;   

DOI  :  10.3390/ma8030867

来源: mdpi

【 摘 要 】

Titanium (Ti) and its alloys are amongst the most commonly-used biomaterials in orthopedic and dental applications. The Ti-aluminum-vanadium alloy (Ti6Al4V) is widely used as a biomaterial for these applications by virtue of its favorable properties, such as high tensile strength, good biocompatibility and excellent corrosion resistance. TiO₂ nanotube (NTs) layers formed by anodization on Ti6Al4V alloy have been shown to improve osteoblast adhesion and function when compared to non-anodized material. In his study, NTs were grown on a Ti6Al4V alloy by anodic oxidation for 5 min using a super-oxidative aqueous solution, and their in vitro biocompatibility was investigated in pig periosteal osteoblasts and cartilage chondrocytes. Scanning electron microscopy (SEM), energy dispersion X-ray analysis (EDX) and atomic force microscopy (AFM) were used to characterize the materials. Cell morphology was analyzed by SEM and AFM. Cell viability was examined by fluorescence microscopy. Cell adhesion was evaluated by nuclei staining and cell number quantification by fluorescence microscopy. The average diameter of the NTs was 80 nm. The results demonstrate improved cell adhesion and viability at Day 1 and Day 3 of cell growth on the nanostructured material as compared to the non-anodized alloy. In conclusion, this study evidences the suitability of NTs grown on Ti6Al4V alloy using a super-oxidative water and a short anodization process to enhance the adhesion and viability of osteoblasts and chondrocytes. The results warrant further investigation for its use as medical implant materials.

【 授权许可】

CC BY   
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