Gelatin Tight-Coated Poly(lactide-co-glycolide) Scaffold Incorporating rhBMP-2 for Bone Tissue Engineering

Materials

Juan Wang²  Dongsong Li⁴  Tianyi Li³  Jianxun Ding¹  Jianguo Liu⁴  Baosheng Li²  Xuesi Chen¹ 

[1] Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; E-Mails:;Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300070, China; E-Mail:;Orthopedics Dept. 2, Heilongjiang Provincial Corps Hospital of Chinese People’s Armed Police Forces, Harbin 150076, China; E-Mail:;Department of Orthopaedic Surgery, the First Hospital of Jilin University, Changchun 130021, China; E-Mail:

关键词: biomaterials;    bone tissue engineering;    gelatin;    surface coating;    recombinant human bone morphogenic protein-2;   

DOI  :  10.3390/ma8031009

来源: mdpi

【 摘 要 】

Surface coating is the simplest surface modification. However, bioactive molecules can not spread well on the commonly used polylactone-type skeletons; thus, the surface coatings of biomolecules are typically unstable due to the weak interaction between the polymer and the bioactive molecules. In this study, a special type of poly(lactide-co-glycolide) (PLGA)-based scaffold with a loosened skeleton was fabricated by phase separation, which allowed gelatin molecules to more readily diffuse throughout the structure. In this application, gelatin modified both the internal substrate and external surface. After cross-linking with glutaraldehyde, the surface layer gelatin was tightly bound to the diffused gelatin, thereby preventing the surface layer gelatin coating from falling off within 14 days. After gelatin modification, PLGA scaffold demonstrated enhanced hydrophilicity and improved mechanical properties (i.e., increased compression strength and elastic modulus) in dry and wet states. Furthermore, a sustained release profile of recombinant human bone morphogenetic protein-2 (rhBMP-2) was achieved in the coated scaffold. The coated scaffold also supported the in vitro attachment, proliferation, and osteogenesis of rabbit bone mesenchymal stem cells (BMSCs), indicating the bioactivity of rhBMP-2. These results collectively demonstrate that the cross-linked-gelatin-coated porous PLGA scaffold incorporating bioactive molecules is a promising candidate for bone tissue regeneration.

【 授权许可】

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