Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain

International Journal of Molecular Sciences

Yuhua Gao²  Xiangchen Li²  Dong Zheng¹  Weijun Guan²  Yuehui Ma² 

[1] College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China; E-Mail:;Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; E-Mails:

关键词: bovine;    neural stem cell;    differentiation;   

DOI  :  10.3390/ijms16035990

来源: mdpi

【 摘 要 】

We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells (NSCs) not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes).

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.