Synthesis of Peptide Nucleic Acids Containing a Crosslinking Agent and Evaluation of Their Reactivities

Molecules

Takuya Akisawa²  Yuki Ishizawa²  Fumi Nagatsugi¹ 

[1] Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-shi, Miyagi 980-8577, Japan;

关键词: peptide nucleic acid (PNA);    crosslink;    antisense;    invasion;   

DOI  :  10.3390/molecules20034708

来源: mdpi

【 摘 要 】

Peptide nucleic acids (PNAs) are structural mimics of nucleic acids that form stable hybrids with DNA and RNA. In addition, PNAs can invade double-stranded DNA. Due to these characteristics, PNAs are widely used as biochemical tools, for example, in antisense/antigene therapy. Interstrand crosslink formation in nucleic acids is one of the strategies for preparing a stable duplex by covalent bond formation. In this study, we have synthesized PNAs incorporating 4-amino-6-oxo-2-vinylpyrimidine (AOVP) as a crosslinking agent and evaluated their reactivities for targeting DNA and RNA.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.