Novel Biochip Platform for Nucleic Acid Analysis

Sensors

Salvatore Pernagallo¹  Giorgio Ventimiglia³  Claudia Cavalluzzo¹  Enrico Alessi³  Hugh Ilyine¹  Mark Bradley² 

[1] DestiNA Genomics Ltd., West Mains Road, Edinburgh EH9 3JJ, UK; E-Mails:;School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK; E-Mail:;STMicroelectronics, ANALOG, MEMS and SENSORS Group, Healthcare BDU, Stradale Primosole 50, Catania 95121, Italy; E-Mail:

关键词: Lab-on-Chip (LoC);    microarray;    nucleic acid;    dynamic chemistry;    polymerase chain reaction (PCR);    peptide nucleic acid (PNA);    microfluidic;    single nucleotide polymorphism (SNP);    microRNA-122 (miRNA122);    mengo virus (MGV);    nucleic acid test (NAT);    point-of-care (POC);   

DOI  :  10.3390/s120608100

来源: mdpi

【 摘 要 】

This manuscript describes the use of a novel biochip platform for the rapid analysis/identification of nucleic acids, including DNA and microRNAs, with very high specificity. This approach combines a unique dynamic chemistry approach for nucleic acid testing and analysis developed by DestiNA Genomics with the STMicroelectronics In-Check platform, which comprises two microfluidic optimized and independent PCR reaction chambers, and a sequential microarray area for nucleic acid capture and identification by fluorescence. With its compact bench-top “footprint” requiring only a single technician to operate, the biochip system promises to transform and expand routine clinical diagnostic testing and screening for genetic diseases, cancers, drug toxicology and heart disease, as well as employment in the emerging companion diagnostics market.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.