Protease and Hemicellulase Assisted Extraction of Dietary Fiber from Wastes of Cynara cardunculus

International Journal of Molecular Sciences

Cinthia Santo Domingo¹  Marcelo Soria²  Ana M. Rojas¹  Eliana N. Fissore¹  L໚ N. Gerschenson¹ 

[1] Industry Department, School of Natural and Exact Sciences, Buenos Aires University (UBA), Ciudad Universitaria, 1428 Ciudad Autónoma de Buenos Aires, Argentina; E-Mails:;Instituto de Investigaciones en Biociencias Agrícolas y Ambientales—INBA (CONICET), School of Agronomy (UBA), 1417 Ciudad Autónoma de Buenos Aires, Argentina; E-Mail:

关键词: Cynara cardunculus;    protease;    hemicellulase;    biomass to chemicals;    inulin;    pectin;    polyphenols;   

DOI  :  10.3390/ijms16036057

来源: mdpi

【 摘 要 】

The action of protease and hemicellulase for the extraction of fractions enriched in soluble fiber from bracts and stems of Cynara cardunculus was evaluated. Using a two-factor simplex design comprising protease amounts of 0–200 μL and hemicellulase amounts of 0–200 mg for 5g of material, we explored the effect of a 5 h enzymatic treatment at 40°C on the chemical composition and yield of the fractions isolated. The fractions contained inulin and pectin. In general, the protein, inulin, and polyphenol contents and also the yields were higher for fractions obtained from stems. The most marked effects were observed when enzymes were used at higher concentrations, especially for hemicellulase. The inclusion of a pre-heating step increased the yield and the inulin content for fractions isolated from bracts and stems and decreased the protein and polyphenol contents, and the galacturonic acid for bracts. These fractions, in general, contained the polyphenolic compounds monocaffeoylquinic acid, apigenin, and pinoresinol.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.