Combined Effect of a Microporous Layer and Type I Collagen Coating on a Biphasic Calcium Phosphate Scaffold for Bone Tissue Engineering

Materials

Mun-Hwan Lee¹  Changkook You¹  Kyo-Han Kim² 

[1] Department of Medical & Biological Engineering, Graduate School, Kyungpook National University, 2-188-1 Samduk-dong, Jung-gu, Daegu 700-412, Korea; E-Mails:;Department of Dental Biomaterials, School of Dentistry, Kyungpook National University, 2-188-1 Samduk-dong, Jung-gu, Daegu 700-412, Korea

关键词: biphasic calcium phosphate;    microporous layer;    type I collagen;    combined effect;    bone tissue engineering;   

DOI  :  10.3390/ma8031150

来源: mdpi

【 摘 要 】

In this study, type I collagen was coated onto unmodified and modified microporous biphasic calcium phosphate (BCP) scaffolds. Surface characterization using a scanning electron microscope (SEM) and a surface goniometer confirmed the modification of the BCP coating. The quantity of the collagen coating was investigated using Sirius Red staining, and quantitative assessment of the collagen coating showed no significant differences between the two groups. MG63 cells were used to evaluate cell proliferation and ALP activity on the modified BCP scaffolds. The modified microporous surfaces showed low contact angles and large surface areas, which enhanced cell spreading and proliferation. Coating of the BCP scaffolds with type I collagen led to enhanced cell-material interactions and improved MG63 functions, such as spreading, proliferation, and differentiation. The micropore/collagen-coated scaffold showed the highest rate of cell response. These results indicate that a combination of micropores and collagen enhances cellular function on bioengineered bone allograft tissue.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.