Resistance Training Regulates Cardiac Function through Modulation of miRNA-214

International Journal of Molecular Sciences

Stéphano Freitas Soares Melo¹  Valério Garrone Barauna²  Miguel Araújo Carneiro Júnior³  Luiz Henrique Marchesi Bozi¹  Lucas Rios Drummond³  Antônio José Natali³  Edilamar Menezes de Oliveira¹ 

[1] Laboratory of Biochemistry and Molecular Biology of the Exercise, School of Physical Education and Sport, University of Sao Paulo, Sao Paulo 05508-030, Brazil; E-Mails:;Laboratory of Molecular Physiology, Health Sciences Center, Federal University of Espírito Santo, Vitória 29043-900, Brazil; E-Mail:;Department of Physical Education, Federal University of Viçosa, Viçosa, Minas Gerais 36570-900, Brazil; E-Mails:

关键词: resistance training;    cardiovascular;    microRNA;    cardiomyocytes;   

DOI  :  10.3390/ijms16046855

来源: mdpi

【 摘 要 】

Aims: To determine the effects of resistance training (RT) on the expression of microRNA (miRNA)-214 and its target in sarcoplasmic reticulum Ca²⁺-ATPase (SERCA2a), and on the morphological and mechanical properties of isolated left ventricular myocytes. Main methods: Male Wistar rats were divided into two groups (n = 7/group): Control (CO) or trained (TR). The exercise-training protocol consisted of: 4 × 12 bouts, 5×/week during 8 weeks, with 80% of one repetition maximum. Key findings: RT increased the left ventricular myocyte width by 15% and volume by 12%, compared with control animals (p < 0.05). The time to half relaxation and time to peak were 8.4% and 4.4% lower, respectively, in cells from TR group as compared to CO group (p < 0.05). RT decreased miRNA-214 level by 18.5% while its target SERCA2a expression were 18.5% higher (p < 0.05). Significance: Our findings showed that RT increases single left ventricular myocyte dimensions and also leads to faster cell contraction and relaxation. These mechanical adaptations may be related to the augmented expression of SERCA2a which, in turn, may be associated with the epigenetic modification of decreased miRNA-214 expression.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.