Cytoprotective Effect of Hispidin against Palmitate-Induced Lipotoxicity in C2C12 Myotubes

Molecules

Jun Myoung Park¹  Jong Seok Lee¹  Jeong Eun Song¹  Ye Chan Sim¹  Suk-Jin Ha¹  Eock Kee Hong¹ 

[1] Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon-do 200-701, Korea; E-Mails:

关键词: hispidin;    oxidative stress;    type 2 diabetes;    C2C12 skeletal muscle cells;    Phellinus linteus;   

DOI  :  10.3390/molecules20045456

来源: mdpi

【 摘 要 】

It is well known that Phellinus linteus, which produces hispidin and its derivatives, possesses antioxidant activities. In this study, we investigated whether hispidin has protective effects on palmitate-induced oxidative stress in C2C12 skeletal muscle cells. Our results showed that palmitate treatment in C2C12 myotubes increased ROS generation and cell death as compared with the control. However, pretreatment of hispidin for 8 h improved the survival of C2C12 myotubes against palmitate-induced oxidative stress via inhibition of intracellular ROS production. Hispidin also inhibited palmitate-induced apoptotic nuclear condensation in C2C12 myotubes. In addition, we found that hispidin can suppress cleavage of caspase-3, expression of Bax, and NF-κB translocation. Therefore, these results suggest that hispidin is capable of protecting C2C12 myotubes against palmitate-induced oxidative stress.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.