Determining the Composition of Lignins in Different Tissues of Silver Birch

Plants

Kurt V. Fagerstedt²  Pekka Saranpää¹  Tarja Tapanila¹  Juha Immanen²  Juan Antonio Alonso Serra²  Kaisa Nieminen¹ 

[1] Natural Resources Institute Finland, Jokiniemenkuja 1, Fi-01300 Vantaa, Finland; E-Mails:;Department of Biosciences, Division of Plant Biology, P.O. Box 65, University of Helsinki, FI-00014 Helsinki, Finland; E-Mails:

关键词: acetyl bromide;    Betula pendula;    cupric oxide;    lignin analysis methods;    phloem;    thioacidolysis;    xylem;   

DOI  :  10.3390/plants4020183

来源: mdpi

【 摘 要 】

Quantitative and qualitative lignin analyses were carried out on material from the trunks of silver birch (Betula pendula Roth) trees. Two types of material were analyzed. First, whole birch trunk pieces were cryosectioned into cork cambium, non-conductive phloem, the cambial zone (conductive phloem, cambium and differentiating xylem), lignified xylem and the previous year’s xylem; material that would show differences in lignin amount and quality. Second, clonal material from one natural birch population was analyzed to show variations between individuals and between the lignin analysis methods. The different tissues showed marked differences in lignin amount and the syringyl:guaiacyl (S/G) ratio. In the non-conductive phloem tissue containing sclereids, the S/G ratio was very low, and typical for phloem fibers and in the newly-formed xylem, as well as in the previous year’s xylem, the ratio lay between five and seven, typical for broadleaf tree xylem. Clonal material consisting of 88 stems was used to calculate the S/G ratios from the thioacidolysis and CuO methods, which correlated positively with an R² value of 0.43. Comparisons of the methods indicate clearly that the CuO method is a good alternative to study the monomeric composition and S/G ratio of wood lignins.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.