NMR Study of the O-Specific Polysaccharide and the Core Oligosaccharide from the Lipopolysaccharide Produced by Plesiomonas shigelloides O24:H8 (Strain CNCTC 92/89)

Molecules

Lena C. E. Lundqvist²  Marta Kaszowska¹  Corine Sandström² 

[1] Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, Wroclaw PL-53-114, Poland; E-Mail:;Department of Chemistry and Biotechnology, Uppsala BioCenter Swedish University of Agricultural Sciences, P.O. Box 7015, Uppsala SE-750-07, Sweden; E-Mail:

关键词: lipopolysaccharide;    Plesiomonas shigelloides;    NMR;    ESI MS;    structural analysis;   

DOI  :  10.3390/molecules20045729

来源: mdpi

【 摘 要 】

The structures of the O-specific polysacccharide and core oligosaccharide of the lipopolysaccharide from Plesiomonas shigelloides O24:H8, strain CNCTC 92/89, have been investigated by NMR spectroscopy and ESI mass spectrometry. The O-specific polysaccharide was found to be composed of a tetrasaccharide repeating unit consisting of [→3)-α-FucpNAc-(1→3)-α-GalpNAcA-(1→3)-α-QuipNAc-(1→] and of α-RhapNAc (1→4) linked to the GalpNAcA residue. An identical structure has been reported for the capsular polysaccharide of the clinical isolate of Vibrio vulnificus strain BO62316 [1]. The core oligosaccharide was composed of a decasaccharide which structure is identical with these in P. shigelloides serotype O54 [2] and serotype O37 [3].

【 授权许可】

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