Structural Studies of the Lipopolysaccharide from the Fish Pathogen Aeromonas veronii Strain Bs19, Serotype O16

Marine Drugs

Anna Turska-Szewczuk³  Katarzyna A. Duda²  Dominik Schwudke⁴  Agnieszka Pekala¹  Alicja Kozinska¹ 

[1] Department of Fish Diseases, National Veterinary Research Institute, Partyzantow 57, Pulawy 24-100, Poland; E-Mails:;Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 4a/c, Borstel D-23845, Germany; E-Mails:;Department of Genetics and Microbiology, M. Curie-Sklodowska University, Akademicka 19, Lublin 20-033, Poland;Division of Bioanalytical Chemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 10, Borstel D-23845, Germany; E-Mail:

关键词: lipopolysaccharide;    O-specific polysaccharide;    Aeromonas veronii;    fish pathogen;    ESI MS;    NMR;   

DOI  :  10.3390/md12031298

来源: mdpi

【 摘 要 】

Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16. ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep₅Hex₃HexN₁Kdo₁P₁. Sugar and methylation analysis together with 1D and 2D ¹H and ¹³C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-d-Quip3NAc-(1→3)-α-l-Rhap-(1→4)-β-d-Galp-(1→3)-α-d-GalpNAc-(1→. This composition was confirmed by mass spectrometry. The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H₂O). Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.