Cellular Anti-Melanogenic Effects of a Euryale ferox Seed Extract Ethyl Acetate Fraction via the Lysosomal Degradation Machinery

International Journal of Molecular Sciences

Seung-Hwa Baek¹  In-Jeong Nam¹  Hyeong Seob Kwak¹  Ki-Chan Kim¹  Sang-Han Lee¹ 

[1] Department of Food Science & Biotechnology, Graduate School, Kyungpook National University, Daegu 702-701, Korea; E-Mails:

关键词: Euryale ferox;    seed;    melanin;    tyrosinase;    lysosomal degradation;   

DOI  :  10.3390/ijms16059217

来源: mdpi

【 摘 要 】

The aim of this study was to investigate the effect of ethyl acetate fraction of Euryale ferox seed extracts (Efse-EA) on melanogenesis in immortalized mouse melanocyte cell line, melan-a. Efse-EA showed strong dose-dependent mushroom tyrosinase inhibitory activity. Treatment of melan-a cells with 30 μg/mL Efse-EA produced strong inhibition of cellular tyrosinase and melanin synthesis. Efse-EA significantly reduced the levels of melanogenesis-related proteins, such as tyrosinase, tyrosinase-related proteins 1 and 2, and microphthalmia-associated transcription factor. Because Efse-EA treatment reduced tyrosinase protein levels without changing its mRNA expression, we investigated whether this decrease was related to proteasomal or lysosomal degradation of tyrosinase. We found that chloroquine, a lysosomal proteolysis inhibitor, almost completely abolished both the down-regulation of tyrosinase and the inhibition of melanin synthesis induced by Efse-EA. These results suggested that Efse-EA may contribute to the inhibition of melanogenesis by altering lysosomal degradation of tyrosinase, and that this extract may provide a new cosmetic skin-whitening agent.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.